I don't know the answer to your question it could be from inbreeding?I hope you can figure out the problem. *hugs*
What breed is she?
I have an animal behavioral specialist lined up for her - if the spayed does not work.....I hope getting her fixed helps...if not try a behaviorist...we used one a few years ago with our crazy dog and now she is amazing lol...
NO.... the two male dogs are not fixed, I never had any trouble between them, still do not - they get along real good....are the other two dogs neutered?